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Amaxa amaxa v buffer
Amaxa V Buffer, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+buffer/amaxa+buffer+v/pm42090422-299-10-10
Average 86 stars, based on 1 article reviews
amaxa v buffer - by Bioz Stars, 2026-09
86/100 stars

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Polymer:

Article Title: The impact of non-electrical factors on electrical gene transfer
Article Snippet: .. The proposed combination of OptiMem buffer and pluronic polymer is also achievable for in vitro stem cell transfer and provides a significant benefit in cost reduction over the Amaxa buffer. ..

In Vitro:

Article Title: The impact of non-electrical factors on electrical gene transfer
Article Snippet: .. The proposed combination of OptiMem buffer and pluronic polymer is also achievable for in vitro stem cell transfer and provides a significant benefit in cost reduction over the Amaxa buffer. ..

Centrifugation:

Article Title: Transformation of Diplonema papillatum, the type species of the highly diverse and abundant marine microeukaryotes Diplonemida (Euglenozoa).
Article Snippet: No. MG490656).The vector was isolated using QIAprep Spin Miniprep Kit (Qiagen, 27106) and either directly used for electroporation or cut with NotI restriction enzyme, isolated from the gel using QIAquick Gel Extraction Kit (Qiagen, 28706), and re-suspended in 10 ll of deionized distilled water before electroporation. .. A total of 5 3 107 cells (2 3 106 cells/ml) was harvested by centrifugation at 1300 g for 10 min at 258C and re-suspended either in 100 ll cytomix buffer (van den Hoff et al., 1992) and electroporated with the BTX machine (1600V, 25X, 50 lF), or in 100 ll of AMAXA buffer (81.8 ll of Human T-cell nucleofector solution 1 18.2 ll of Supplement) for the electroporation by Amaxa Nucleofector II. ..

Article Title: Numb regulates Tau levels and prevents neurodegeneration in tauopathy mouse models.
Article Snippet: .. For RGC transfection, cells were resuspended in Amaxa buffer and supplemented with 2 g of DNA (Numb-72 was cloned into pCIG-IRES-GFP; GFP was empty vector; and Tau::GFP and Tau::P301L were gifts from N. Leclerc, Université de Montréal) after centrifugation. .. Then, cells were transferred in an electroporation cuvette, and nucleofection was carried out according to manufacturer’s recommendation (Amaxa nucleofector, program #33, Lonza Technology).

Electroporation:

Article Title: Transformation of Diplonema papillatum, the type species of the highly diverse and abundant marine microeukaryotes Diplonemida (Euglenozoa).
Article Snippet: No. MG490656).The vector was isolated using QIAprep Spin Miniprep Kit (Qiagen, 27106) and either directly used for electroporation or cut with NotI restriction enzyme, isolated from the gel using QIAquick Gel Extraction Kit (Qiagen, 28706), and re-suspended in 10 ll of deionized distilled water before electroporation. .. A total of 5 3 107 cells (2 3 106 cells/ml) was harvested by centrifugation at 1300 g for 10 min at 258C and re-suspended either in 100 ll cytomix buffer (van den Hoff et al., 1992) and electroporated with the BTX machine (1600V, 25X, 50 lF), or in 100 ll of AMAXA buffer (81.8 ll of Human T-cell nucleofector solution 1 18.2 ll of Supplement) for the electroporation by Amaxa Nucleofector II. ..

Article Title: Competitive electroporation formulation for cell therapy
Article Snippet: We determined the relative efficiency based on the amount of gfp production expressed as a relative percentage of the amount of luciferase expressed by the same cells transfected using the Amaxa formulation ( ). .. As illustrates, our formulation outperforms Amaxa buffer in electroporation transfection efficiency in five out of ten cell lines. fig ft0 fig mode=article f1 ..

Incubation:

Article Title: Molecular basis of VEGFR1 autoinhibition at the plasma membrane
Article Snippet: Alternatively, RAW264.7 cells were nucleofected using the D032 program in the Lonza nucleofector. .. 4 × 10 7 cells were incubated with 4 μg of DNA in Amaxa buffer for 5 min at room temperature. ..

Transfection:

Article Title: Stem Cells Expanded from the Human Embryonic Hindbrain Stably Retain Regional Specification and High Neurogenic Potency
Article Snippet: Plasmids were transfected using the Amaxa or Neon nucleofection kits according to the manufacturer's protocol. .. For nucleofection with Amaxa, 4 × 10 6 NES cells were transfected with 2 μg of Piggybac plasmid DNA together with 2 μg pBase (transposase) in 100 μl of Amaxa buffer. .. The mixture was added to Amaxa cuvettes before nucleofection using the T20 program (Amaxa).

Article Title: Numb regulates Tau levels and prevents neurodegeneration in tauopathy mouse models.
Article Snippet: .. For RGC transfection, cells were resuspended in Amaxa buffer and supplemented with 2 g of DNA (Numb-72 was cloned into pCIG-IRES-GFP; GFP was empty vector; and Tau::GFP and Tau::P301L were gifts from N. Leclerc, Université de Montréal) after centrifugation. .. Then, cells were transferred in an electroporation cuvette, and nucleofection was carried out according to manufacturer’s recommendation (Amaxa nucleofector, program #33, Lonza Technology).

Article Title: Competitive electroporation formulation for cell therapy
Article Snippet: We determined the relative efficiency based on the amount of gfp production expressed as a relative percentage of the amount of luciferase expressed by the same cells transfected using the Amaxa formulation ( ). .. As illustrates, our formulation outperforms Amaxa buffer in electroporation transfection efficiency in five out of ten cell lines. fig ft0 fig mode=article f1 ..

Plasmid Preparation:

Article Title: Stem Cells Expanded from the Human Embryonic Hindbrain Stably Retain Regional Specification and High Neurogenic Potency
Article Snippet: Plasmids were transfected using the Amaxa or Neon nucleofection kits according to the manufacturer's protocol. .. For nucleofection with Amaxa, 4 × 10 6 NES cells were transfected with 2 μg of Piggybac plasmid DNA together with 2 μg pBase (transposase) in 100 μl of Amaxa buffer. .. The mixture was added to Amaxa cuvettes before nucleofection using the T20 program (Amaxa).

other:

Article Title: Intracellular genomic transplant and methods of therapy
Article Snippet: For example, TCR transgene sequence of MBVb22 will be acquired and synthesized by IDT as a gBLOCK.

Clone Assay:

Article Title: Numb regulates Tau levels and prevents neurodegeneration in tauopathy mouse models.
Article Snippet: .. For RGC transfection, cells were resuspended in Amaxa buffer and supplemented with 2 g of DNA (Numb-72 was cloned into pCIG-IRES-GFP; GFP was empty vector; and Tau::GFP and Tau::P301L were gifts from N. Leclerc, Université de Montréal) after centrifugation. .. Then, cells were transferred in an electroporation cuvette, and nucleofection was carried out according to manufacturer’s recommendation (Amaxa nucleofector, program #33, Lonza Technology).

Formulation:

Article Title: Competitive electroporation formulation for cell therapy
Article Snippet: We determined the relative efficiency based on the amount of gfp production expressed as a relative percentage of the amount of luciferase expressed by the same cells transfected using the Amaxa formulation ( ). .. As illustrates, our formulation outperforms Amaxa buffer in electroporation transfection efficiency in five out of ten cell lines. fig ft0 fig mode=article f1 ..



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